Study on Anti-tumor Activities of Physalins B in Vitro / 广州中医药大学学报

ABSTRACT

Objective To study the in-vitro anti-tumor activities of physalin B (PhB) extracted from Physalis angulata L.. Methods Th e effect of PhB on the cell proliferation and the morphology changes of tumor cell strains HepG2 and SGC7901 was observed by methyl thiazolyl tetrazolium (MTT) assay and by phase contrast microscopy respectively, and the results were compared to positive control anti-cancer drug hydroxycamptothecine (HCTP) . Apoptosis of HepG2 and SGC7901 cells induced by PhB was determined by 4’ , 6-diamidino-2-phenylindole ( DAPI) staining method. Results MTT results showed that the growth of HepG2 and SGC7901 was significantly inhibited by PhB in dose-and time-dependent manner, and the inhibitory effect of high concentration of PhB was better than the positive control medicine HCTP. Phase contrast microscopy results showed that the cellular morphology was improved by PhB, which was accorded with the results showed by MTT assay. DAPI staining results showed that the apoptosis of HepG2 and SGC7901 was enhanced by PhB. Conclusion PhB extracted from Physalis pubescens L. exerts certain inhibitory effect on the proliferation of HepG2 and SGC7901 cells.