Expression of pyrrole drugs resistance genes in Candida albicans from genital tract / 国际检验医学杂志

ABSTRACT

Objective To explore the expression of resistance genes in pyrrole resistant Candida albicans from genital tract . Methods The Candida albicans were isolated from the vaginal secretions of 93 vaginitis patients ,and Kirby-Bauer method was per-formed for preliminary culture of strains resistant .The total RNA was extracted from pyrrole-resistant and susceptible Candida al-bicans isolated by pyrolysis method and cDNA was synthesized .The expression of CDR1 ,CDR2 ,MDR1 genes was then detected by Real-time quantitative PCR .Results A total of 59 strains of Candida albicans ,18 strains of Candida tropicalis ,10 strains of Candida krusei and 6 strains of Candida glabrata were cultured .37 strains of Candida albicans were resistant to fluconazole ,ketoconazole ,and miconazole at different degrees ,and the drug resistance rates were 16 .7% ,18 .3% ,51 .7% ;CDR1 gene′s 2 -△ △ Ct in sensitive strains group was 1 .09 ± 0 .27 ,while resistant strains group was 1 .46 ± 0 .24 ,there was significant difference between two groups (t =- 4 .22 ,P= 0 .001) .There was no significant difference in the expression of CDR2 and MDR1 genes between the sensitive and re-sistant strains(P= 0 .170 ,P= 0 .800) .Conclusion The drug resistance of Candida albicans mechanism is complicated ,it might be associated with high CDR1 expression ,the relationship between CDR2 ,MDR1 expression and multidrug resistance needs further study .