Determination of the concentration of ziprasidone in human plasma by using RP-HPLC / 国际检验医学杂志

ABSTRACT

Objective To develop a method for determining the concentration of ziprasidone in human plasma by using HPLC . Methods The drug concentration of plasma was analyzed in a reverse phase HPLC system C18 column(250 mm × 4 .6 mm ,5 μm);mobile phase consisted of 0 .2% ammonium triethyamine‐methanol(15∶85);the flow rate was1 .1 mL/min;the column temperature was 35°C .The detection wavelength was at 230 nm .Ethyl acetate was used as extracting solvent .Results A good linearity range was 8 .0-300 .0 ng/mL concentration of ziprasidone in plasma .The average recoveries of ziprasidone in low ,middle and high con‐centrations(8 .0 ,80 .0 ,350 .0 ng/mL) over 95% respectively ;the extraction recovery over 90% .The intra‐day and inter‐day varia‐tion(RSD)was less than10% (n=5) .The minimum detectable concentration of ziprasidone was 5 ng/mL .The regression equation was Y=0 .089 9X+0 .021 5 .r=0 .999 7(n=7) .Conclusion The method is fast ,accurate ,,sensitive and simple for clinical monito‐ring of ziprasidone in plasma .