Analysis of the Molecular Weight of Peptides in Thymosin by Tricine-SDS-PAGE / 现代检验医学杂志
Journal of Modern Laboratory Medicine
; (4): 132-133,137, 2016.
Article
in Zh
| WPRIM
| ID: wpr-603625
Responsible library:
WPRO
ABSTRACT
Objective To establish a simple and feasible method in analyzing the molecular weight distribution of peptides in thymosin.Methods Improved Tricine-SDS-PAGE electrophoresis was applied to detect the molecular weight of peptides and the content of thymosinα1 in thymosin.Results Tested the thymosin preparations on the domestic market using this im-proved method.It was demonstrated that the peptide molecular weight distribution in thymosin preparations was between 3.5~8.5 kD,also could detect the concentration of 1 μg thymosinα1 in thymosin by using this improved method.Conclusion This improved method is suitable for the analysis of peptides molecular weight distribution and the concentration of thy-mosinα1,so it can be used for control quality of thymosin preparations.
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Modern Laboratory Medicine
Year:
2016
Type:
Article