Effects of interleukin-34 on the proliferation and chemokines expression of fibroblast-like synoviocytes in patients with rheumatoid arthritis / 中华风湿病学杂志

ABSTRACT

Objective To investigate the effects of interleukin (IL)-34 on the proliferation and chemokines expression of fibroblast-like synoviocytes (FLS) in patients with rheumatoid arthritis (RA).Methods RA FLS were isolated and cultured in the presence or absence of IL-34.FLS proliferation was determined by methyl thiazlyl tetrazoliu method (MTT) method.The levels of chemokines from RA FLS supernatant were detected by protein chip AAH-CYT-G1000 assay.The IL-34 receptor (IL-34R) expression on RA FLS was analyzed by flow cytometry (FCM).Statistical analysis between groups was performed by t test.Results Compared to the control group, the proliferation of IL-34-stimulated RA FLS was obviously increased, and up to the maximum at 72 hours.In addition, the levels of chemokines [epithelial neutrohil-activating peptide 78 (ENA-78), IL-8, growth-related oncogene (GRO), macrophage chemoattractant protein-1 (MCP-1)] on RA FLS supernatant in IL-34 stimulated group were significantly elevated than those in the control group [ENA-78 (397.1±8.2) pg/ml, (54.0±2.9) pg/ml (t=127.61, P＜0.01);IL-8(2 017±36) pg/ml, (778±102) pg/ml (t=36.67, P＜0.01);GRO (4 935±160) pg/ml, (2 746±188) pg/ml (t=43.60, P＜0.01);MCP-1 (46 798±1 293) pg/ml, (27 813±2 329) pg/ml (t=22.43, P＜0.01)].IL-34R was highly expressed on the RA FLS.Conclusion IL-34 promotes RA FLS proliferation and chemokines expression, which may be one of the important mechanisms involved in the pathogenesis of RA.