Protective effect of diacerein on MIA-induced injury in rat osteoarthritis chondrocytes / 重庆医学

ABSTRACT

Objective To investigate the protective effect of diacerein on monosodium iodoacetate (MIA) induced injury in rat osteoarthritis chondrocytes .Methods The experiment was divided into five groups ,including the normal group ,model group (4μM MIA) ,diacerein low ,middle and high doses groups (1 ,10 ,100μM) .The viability of chondrocytes was detected by MTT assay . The activity of cysteinyl aspartate specific proteinase‐3 (Caspase‐3) was measured by spectrophotography .The activation of nuclear factor kappa B (NF‐κB) signaling pathway and expression level of downstream target molecule cell Bax ,Bcl‐2 ,matrix metalloprotei‐nase‐9 (MMP‐9) and MMP‐13 were detected by Western blot .Results 1 ,10 ,100μM diacerein could increase the viability of MIA‐induced chondrocytes and reduce the activity of Caspase‐3(P<0 .05) .10 ,100μM diacerein could decrease the phosphorylation level of IκBαand NF‐κB p65 ,furthermore downregulated the level of Bax ,MMP‐9 and MMP‐13 protein ,and upregulated the level of Bcl‐2 protein (P<0 .05) .Conclusion Diacerein could inhibit cell apoptosis and degradation of extracellular matrix in MIA‐induced rat chondrocytes ,which might be related to the NF‐κB signal pathway .