Effects of siRNA silencing of inhibitor of apoptosis protein gene on the proliferation of airway smooth muscle in bronchial asthma mice / 重庆医学

ABSTRACT

Objective To investigate the effects of siRNA silencing of inhibitor of apoptosis protein gene on the proliferation of airway smooth muscle in bronchial asthma mice .Methods Tweenty female BALB/c mice were randomly divided into experimen‐tal group and control group .Mice in the experimental group were constructed asthma models .The trachea and bronchi were collect‐ed for cell culture .Respectively ,the cultured airway smooth muscle cells in the experimental group and the control group were groupedPBS control group (added to PBS solution) ,missense chain control group (transfected with missense chain) and siRNA transfection group (transfected with siRNA Survivin) .The expressions of Survivin mRNA ,Survivin protein and caspase‐9 protein in airway smooth muscle cells were detected .The levels of IL‐6 and CCL5 in cell supernatant were detected .Results RT‐PCR re‐sults showed that ,the relative expression levels of Survivin RNA of airway smooth muscle cells in the siRNA transfection group of the experimental group were lower than the PBS control group and missense chain group ,the differences were statistically signifi‐cant (P<0 .05) .Western blot results showed that ,the expressions of Survivin proteins in the siRNA transfection group of the ex‐perimental group were lower than the PBS control group and missense chain control group ,while the caspase‐9 protein were higher , In the experimental group ,the synthesis and secretion levels of IL‐6 and CCL5 of airway smooth muscle cells in the siRNA transfec‐tion group were lower than the PBS control group and missense chain control group ,the cell proliferations at 2-4 d in the siRNA transfection group were lower than the PBS control group and missense chain control group ,the proportion of G0/G1 phase in the siRNA transfection group were higher than the PBS control group and missense chain control group ,but the proportion of S/G2 phase were lower than the PBS control group and missense chain control group ,cells apoptosis rates of the siRNA transfection group were lower than the PBS control group and missense chain control group ,the differences were statistically significant (P<0 .05) .Conclusion siRNA specific silencing Survivin gene could accelerate the airway smooth muscle cell apoptosis ,and inhibit cell abnormal proliferation and secretion .