Separation and Analysis of Drug Resistance of Staphylococcus Aureus in Patients with Respiratory Infection of Children / 现代检验医学杂志

ABSTRACT

Objective Discuss and analyze Staphylococcusaureus in isolation and drug resistance in patients with pediatric re-spiratory infection,providing guidelines for clinical treatment on the control of infection.Approaches collect 5 413 cases of respiratory tract infection from Jan.2013 to Dec.2014 in patients (including pediatrics neonatology)with sputum specimens, applying MicroScan-As4 automated Microbes Identification analyzer to identify and test drug sensitiveness of the separated strains,using Whonet 5.6 for statistical analysis.Results 1 540 strains of 29 different pathogenic bacteria were isolate from pediatric patients with respiratory tract infection,among which there were 336 strains (21.82%,336/1540)of Staphylococ-cus aureus (SAU,S.aureus),more than 1/5 of the total of pathogenic bacteria.And the isolation rate of pediatric respiratory tract infection accounted for 6.2 1%,significantly higher than that of other pathogenic bacteria isolation rate (with the excep-tion of Haemophilus influenzae>5%,the rest all<5%).In all detected SAU,methicillin-resistant Staphylococcus aureus (MRSA)occpied 59 strains (17.56%,59/336),which indicated that SAU played a leading place in patients with pediatric respiratory infection.The antimicrobial drug resistance rate of SAU against vancomycin,Nai thiazoleamine,daptomycin,and Quinupristin was 0,whereas to other antibiotics it showed different degrees of resistance.The drug resistance rate of MRSA to penicillin and ampicillin,oxacillin,ampicillin/sulbactam,amoxicillin/clavulanic acid and cefazolin,ceftriaxone was 100%,to erythromycin,clindamycin over 50%,to other antimicrobial resistance in low resistance.Conclusion There were more SAU in children with respiratory infection;especially MRSA bears multi drug resistance.Therefore,the monitoring of drug resist-ance of Staphylococcus aureus should be strengthened,and antimicrobial drugs should be rationally chosen according to the results of drug sensitive test for individuals,so that resistant strains can be under effective control and kept being reduced from emergence.