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In vitro culture and migration assay of mouse smooth muscle progenitor cells / 中国药理学通报
Chinese Pharmacological Bulletin ; (12): 915-919,920, 2016.
Article in Chinese | WPRIM | ID: wpr-604381
ABSTRACT
Aim To establish a reliable method for the culture of mouse smooth muscle progenitor cells and in-vestigate their migration ability .Methods Mesenchy-mal stem cells from compact bones were obtained from C57 BL/6 mice and stimulated with PDGF-BB to in-duce these cells to differentiate into smooth muscle pro-genitor cells. Morphological analysis , immunocyto-chemical and flow cytometric analysis were used to i-dentify the cell type and the migration ability was in-vestigated by the transwell system and flow cytometry . Result After PDGF-BB stimulation for 7 days, the cells showed spindle shape and started to express α-SMA as demonstrated by immunocytochemistry .After 21 days induction , Flow cytometric analysis revealed that over 70%of the cells expressed both CD 34 andα-SMA and 58.5%of the cells expressed SM-MHC.Mi-gration assay showed that the smooth muscle progenitor cells from culture could migrate in vivo and in vitro. Conclusions The culture of smooth muscle progenitor cells from compact bone-derived mesenchymal stem cells is easily operated with high yield rate and shorten culture period . Obtained smooth muscle progenitor cells from culture could migrate in vivo and in vitro, which is suitable for the mechanism studies .

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Pharmacological Bulletin Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Pharmacological Bulletin Year: 2016 Type: Article