Study on correlation between ankylosing spondylitis and HLA -B27 subgenotype / 国际检验医学杂志

ABSTRACT

Objective To explore the correlation between different HLA‐B27 subgenotype and ankylosing spondylitis (AS ) . Methods The whole venous blood was collected from the outpatients and inpatients of the orthopedics ,acupuncture and rheuma‐tism departments and HLA‐B27 was qualitatively detected by using the gene analysis method .Among them ,380 cases of AS were HLA‐B27 positive ,and 50 cases of HLA‐B27 positive were selected as the healthy control group .Then the HLA‐B27 subgenotypes were detected by using the sequence specific primers PCR (PCR‐SSP) technology .Results Among 380 cases of AS ,217 cases (57 .1% ) of HLA‐AS B2704 subgenotype ,143 cases (37 .6% ) of HLA‐B2705 subgenotype ,11 cases (2 .9% ) of HLA‐B2707 sub‐genotype and 9 cases (2 .4% ) of HLA‐B2711 subgenotype were detected out ;among 50 cases of HLA‐B27 positive in the healthy control group ,23 cases (46 .0% ) of HLA‐B2706 subgenotype ,21 cases (42 .0% ) of HLA‐B2709 subgenotype ,4 cases (8 .0% ) of HLA B2704 subgenotype and 2 cases (4 .0% ) of HLA‐B2705 subgenotype were detected out ,the differences between the two groups were statistically significant (P=0 .002) .Conclusion The subgenotypes of HLA‐B27 among the AS patients in Baoji area are dominated by the genotype B2704 and B2705 ,which is strongly correlated with the occurrence of AS among Han population in Baoji area ;B2706 and B2709 are the protective subgenotypes in this area .