Effect of glutamine on p38MAPK and IL-8 expression in PBMC of patients with chronic obstructive pulmonary disease / 重庆医学

ABSTRACT

Objective To investigate the effects of glutamine (Gln) on the p38MAPK activity and IL‐8 expression in periph‐eral blood mononuclear cells(PBMC) of the patients with chronic obstructive pulmonary disease(COPD) .Methods Thirty‐two pa‐tients with acute exacerbation of COPD (AECOPD ) were chosen as the research subjects(AECOPD group) .Then the patients con‐verting to the stable stage of COPD after treatment were set as the SCOPD group .PBMC of each group were extracted and conduc‐ted the Gln intervention .At the same time 16 healthy people were selected as the healthy control group .The real‐time PCR(RT‐PCR) was used to detect the levels of p38MAPK and IL‐8 expression in PBMC among the five groups .Results (1)The expressions of p38MAPK and IL‐8 in the AECOPD blank control group and the SCOPD blank control group were both higher than those in the healthy control group (P<0 .01 or P<0 .05) ,while the expression levels of p38MAPK and IL‐8 in patients with AECOPD was higher than those of patients with SCOPD ,the difference was statistically significant (P< 0 .05) .(2)The expression levels of p38MAPK and IL‐8 in the AECOPD Gln group were lower than those in the AECOPD blank control group (P<0 .01);the expres‐sion levels of p38MAPK and IL‐8 in the SCOPD Gln group were lower than those in the SCOPD blank control group (P<0 .05) . Conclusion Gln could inhibit the p38MAPK pathway activation in inflammatory cells of COPD patients and reduce the expression level of IL‐8 .