Analysis of antibacterial activity and mechanism of polypyridyl ruthenium complex / 中国药理学通报

ABSTRACT

Aim To analyze the antibiotic activity and mechanism of a polypyridyl ruthenium complex. Meth-ods The antibacterial activity of [ ( Phen ) 2 Ru ( dp-pz) ] ( PF6 ) 2 was determined by MIC and MBC value. Based on a fluorescent activity of this complex, the flu-orescent emission spectra was used to analyze the com-bination of complex to DNA. Then the competition combination was analyzed between complex and Gold View to DNA. Lastly, gel electrophoresis of DNA was applied to detect the combination situation between complex and DNA. Results This kind of polypyridyl ruthenium complex showed a significant antibacterial activity with a minimum antibacterial conentration of 0. 2~0. 4 g · L-1 . That was caused by the combina-tion and distortion of DNA due to the activity of this complex. Conclusion The antibacterial activity and the mechanism of antibacterial activity about [ ( Phen) 2 Ru( dppz) ] ( PF6 ) 2 are confirmed in this re-search, which provides a good foundation for the devel-opment of such class of compound.