Preliminary research in relationship between TNFAIP8 and gastric cancer drug resistance / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 962-964,969, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-604573
ABSTRACT
Objective:
To explore if there was a correlation between TNFAIP8 and gastric tumor drug resistance.Methods:
We collected 47 cases of neoadjuvant chemotherapy,and detected the expression of TNFAIP8 in their tissues before and after surgery by im-munohistochemistry. Compared the expression of TNFAIP8 between SGC7901 cell and its cisplatin resistance cell SGC7901-DDP by Western blot,and then we used siRNA technology to decreased its expression,continued to test the susceptibility to cisplatin through MTT.Results:
The expression of TNFAIP8 between pre-neoadjuvant and after-neoadjuvant had no difference, but the expression of chemotherapy effectiveness was lower than chemotherapy invalidness(P<0. 05);the expression of SGC7901-DDP cell was also higher than SGC7901 cell; the expression of TNFAIP8 decreased apparently through plasmid transfection of SGC7901-DDP cell, MTT test showed the fewer expression of TNFAIP8 could increase the susceptibility to cisplatin(P<0. 05).Conclusion:
TNFAIP8 was involved in chemotherapy resistance of gastric carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2016
Type:
Article
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