Determination of Paraquat in Human Plasma by Solid Phase Extraction-HPLC / 中国药房
China Pharmacy
;
(12): 4080-4081,4082, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-605056
ABSTRACT
OBJECTIVE:
To develop a method for the determination of paraquat in human plasma,and to provide experimen-tal evidence for the therapy and prognosis of paraquat-poisoned patients.METHODS:
The human plasma samples were processed using Waters Oasis solid phase extraction column. HPLC determination was performed on DiamonsilTM C18 chromatographic column with mobile phase consisted of 0.1 mol/L phosphate buffer(containing 80 mmol/L sodium heptanesulfonate,pH adjusted to 3.0 by triethylamine)-acetonitrile (82∶18,V/V) at the flow rate of 0.9 ml/min. The detection wavelength was set at 258 nm.RESULTS:
The linear range of paraquat were 20-5 000 ng/ ml;RSDs of inter-day and intra-day both were lower than 9%;average extraction recoveries were 90.72%-96.34%,and average method recoveries were 100.32%-103.10%.CONCLUSIONS:
The solid phase ex-traction HPLC can determine the content of paraquat in human plasma rapidly and accurately.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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