Content Determination of Ferulic Acid in Tianwang Buxin Pills by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method to determine the content of ferulic acid in Tianwang buxin pills. METHODS:HPLC was performed on the column of Diamonsil C18 with the mobile phase of acetonitrile-1% glacial acetic acid solution(15∶85, V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 323 nm,the temperature was room temperature,and the volume was 20 μl. RESULTS:The linear range of ferulic acid was in the range of 0.091 2-2.28 μg/ml(r=0.999 8);the RSDs of precision, stability and reproducibility tests were less than 2.0%,and the average recovery was 98.96%(RSD=0.26%,n=9). CONCLU-SIONSThe method is simple,accurate and reliable,and can be used as the quality control method of ferulic acid in Tianwang buxin pills.