Establishment of the Method for Testing Leguminosae in Qizhu Bishi Granules / 中国药房
China Pharmacy
;
(12): 2562-2563,2564, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-605123
ABSTRACT
OBJECTIVE:
To establish a method for the identification and content determination of leguminosae in Qizhu bishi granules.METHODS:
Thin layer chromatography (TLC) was used to identify the leguminosae. HPLC-ELSD was used to deter-mine the content of astragaloside A in leguminosae. The column was Diamonsil C18 with the mobile phase of acetonitrile-water(32∶68,V/V)at the flow rate of 1.0 ml/min,the column temperature was 40 ℃. The sample size was 20 μl. The drift tube temperature was 108 ℃ with the nitrogen gas flow rate of 2.8 ml/min.RESULTS:
The TLC features of leguminosae were obvious with clear spots and good specificity. Astragaloside A had a good linear relationship in the range of 0.402 4-4.02 μg(r=0.997 0). RSDs of pre-cision,stability and reproducibility tests were≤0.8%. The average recovery was 99.00%(RSD=1.29%,n=9).CONCLUSIONS:
The method is simple,accurate and reliable and can be used for the quality control of Qizhu bishi granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS