Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection
The Korean Journal of Parasitology
;
: 49-54, 2006.
Article
in English
| WPRIM
| ID: wpr-60516
ABSTRACT
In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmodium falciparum
/
Plasmodium vivax
/
Recombinant Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Molecular Sequence Data
/
Base Sequence
/
DNA, Protozoan
/
Amino Acid Sequence
/
Malaria, Falciparum
/
Cloning, Molecular
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2006
Type:
Article
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