Analysis of the Content of Water-soluble Proteins in Euryale ferox from Different Regions / 中国药房

ABSTRACT

OBJECTIVE:To analyze the content of water-soluble proteins in Euryale ferox from different regions. METHODS:The water-soluble proteins in E. ferox were extracted by cryogenic grinding. With the reference of bovine albumin,Coomassie bril-liant blue G-250 was used for coloration,visible spectrophotometry was conducted to determine the content of water-soluble pro-teins in E. ferox from different regions and clustering analysis was used to analyze the results. RESULTS:The linear range of bo-vine albumin was 0.005 01-0.150 3 mg/ml(r=0.999 2)with the average recovery of 98.95%(RSD=1.76%,n=6).RSDs of preci-sion,stability and reproducibility tests were no more than 2.04%. The average value of content of water-soluble proteins in samples was 0.457 7 mg/g. Cluster analysis showed that the content of water-soluble proteins in E. ferox from Yangtze River basin was high-er,followed by the southward and the northward was the least. CONCLUSIONS:The method is simple,reliable and reproducible, and suitable for the content determination of water-soluble proteins in E. ferox.