Study on the HPLC Fingerprint of Abrus mollis Seed / 中国药房

ABSTRACT

OBJECTIVE:To establish the HPLC fingerprint of Abrus mollis seed. METHODS:HPLC was performed on the column of Inertsil ODS-3 C18 with mobile phase of acetonitrile-0.1% phosphoric acid(gradient elution)at flow rate of 1.0 ml/min, detection wavelength was 270 nm,column temperature was 30 ℃ and volume injection was 10 μl. With the reference of abrine, 10 batches of A. mollis seed were analyzed,and similarity evaluation was performed by using chromatographic fingerprint of TCM. RESULTS:There were totally 12 common peaks,and the similarity was more than 0.9. According to the verification,the finger-print of A. mollis seed has good consistency with the reference fingerprint. CONCLUSIONS:The established fingerprint can pro-vide reference for the identification and quality evaluation of A. mollis seed.