Optimization of the Extraction Technology for Oligosaccharides of Morinda officinalis by Response Surface Methodology / 中国药房
China Pharmacy
;
(12): 4847-4849,4850, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-605245
ABSTRACT
OBJECTIVE:
To optimize the extraction technology of oligosaccharides of Morinda officinalis.METHODS:
The liq-uid/solid ratio,extraction time and temperature were chosen as factors,and the yield of oligosaccharides was estimated as index. On the basis of single-factor experiments,using a 3-factor,3-level Box-behnken central composite experimental design,two-poly-nomial regression equation of extraction rate of oligosaccharide was established,and it was analyzed by response surface methodolo-gy to obtain the optimum extraction conditions,and the verification test was conducted.RESULTS:
The optimum extraction condi-tions were as follows as material/liquid ratio of 23∶1(ml/g),extraction time of 1.7 h,and extraction temperature of 93℃,extract-ing for twice. Under these conditions, the estimated and observed average values of extraction rate of oligosaccharides were 10.37% and 10.29%(RSD=0.20%,n=3),respectively. The deviation value was 0.06%.CONCLUSIONS:
The response surface methodology can be used to optimize the extraction process of oligosaccharides of M. officinalis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS