Contents Determination of Chlorogenic Acid and Vitexin in Lophatherum gracile by HPLC / 中国药房
China Pharmacy
;
(12): 833-834,835, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-605264
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophather-um gracile.METHODS:
HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile- water (gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 280 nm,column temperature was 35 ℃,and the injec-tion volume was 10 μl.RESULTS:
The linear range was 0.041 0-1.228 8 μg for chlorogenic acid(r=0.999 8)and 0.264 0-7.920 0μg for vitexin(r=0.999 9);RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.6%-102.3%(RSD=1.85%,n=9) and 97.1%-101.3%(RSD=1.19%,n=9),respectively.CONCLUSIONS:
The method is simple,stable and reproducible,and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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