Simultaneous Determination of the Contents of Geniposide and Liquiritin in Xinshenning Tablets by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for simultaneous determination of the contents of geniposide and liquiritin in Xinshenning tablets. METHODS:HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water (gradient elution) at flow rate of 1.0 ml/min,the column temperature was 25 ℃,detection wavelength was 238 nm and the vol-ume was 10 μl. RESULTS:The mass concentration range was 16.3-407.9 μg/ml for geniposide(r=0.999 9) and 7.4-185.9 μg/ml for liquiritin(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.63%;average recoveries were 99.05%(RSD=1.28%,n=9)and 98.00%(RSD=1.84%,n=9),respectively. CONCLUSIONS:The established method is accu-rate,sensitive and simple,and can be used for the contents determination of geniposide and liquiritin in Xinshenning tablets.