Expression of Human CyclinD1 in Pichia Pastoris / 现代检验医学杂志

ABSTRACT

Objective To obtain the Cyclin D1,the Cyclin D1 gene was cloned and expressed in Pichia pastoris.Methods The total RNA was extracted from liver cancer tissue.The Cyclin D1 cDNA was obtained by RT-PCR.The Cyclin D1 cDNA was sequenced.And sub-clonedto the Ppic9k,the Pichia pastoris expressed was used to obtain the Cyclin D1.Results The 483 bp Cyclin D1 cDNA was obtained.And the sequence of Cyclin D1 was corrected.The 36KD Cyclin D1 was obtained by Pichia pastoris expression.Conclusion The Cyclin D1 cDNA was cloned and Cyclin D1 was expressed in Pichia pastoris.