Concentration Determination of Voriconazole in Human Plasma by UPLC-MS/MS and Its Clinical Application / 中国药房
China Pharmacy
;
(12): 4064-4066,4067, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-605481
ABSTRACT
OBJECTIVE:
To develop a method for concentration determination of voriconazole in human plasma and apply it in the clinic.METHODS:
UPLC-MS/MS method was adopted. Using ketoconazole as internal standard,the determination was per-formed on Shim-pack VP-ODS column with mobile phase consisted of water(containing 1‰ formic acid and 2 mmol/L ammonium acetate)-acetonitrile(gradient elution)at flow rate of 0.3 ml/min and column temperature of 40℃.The electrospray ion source,pos-itive ionizing pattern and multiple reaction monitoring were used;the mass transition ion-pairs of voriconazole and internal standard were m/z 351.2→282.2 and m/z 532.1→490.2.RESULTS:
The linear range of voriconazole were 1-10 000 ng/ml (r=0.999 5,n=5),and the limit of quantitation was 1 ng/ml;RSDs of inter-day and intra-day were all lower than 10%;method recovery was higher than 90%(RSD<8%),and extraction recovery was higher than 70%(RSD<8%). The plasma concentrations of voriconazole in 10 patients with invasive fungal infection determined by this method were 507.33-7 011.24 ng/ml,and those of 3 patients were outside the recommended treatment concentration range.CONCLUSIONS:
The established method is fast,accurate and sensitive,and can be applied for the therapeutic drug monitoring of voriconazole.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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