Content Determination of Amygdalin in the Lianhua Qingwen Capsule by HPLC / 中国药房
China Pharmacy
;
(12): 2978-2979,2980, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-605750
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of amygdalin in Lianhua qingwen capsule. METH-ODSHPLC was performed on the column of Phenomenex Kinetex XB-C18 with mobile phase of acetonitrile-0.2%Phosphoric acid so-lution(6∶94,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 207 nm,column temperature was 30℃,and the injec-tion volume was 10μl.RESULTS:
The linear range of amygdalin was 43.16-215.80 μg/ml(r=0.999 7);the limit of detection was 0.431 6μg/ml,the limit of quantitation was 1.294 8μg/ml;RSDs of precision,stability and reproducibility tests no more than 0.69%;recovery was 95.16%-100.49%(RSD=1.67%,n=9).CONCLUSIONS:
The method is simple and rapid with high accuracy and well reproducibility,and can be used for the content determination of amygdalin in Lianhua qingwen capsule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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