Effects of hesperidin on inflammatory cytokine levels in chronic bronchi-tic rats / 中国病理生理杂志

ABSTRACT

AIM:To observe the effects of hesperidin on the inflammatory cytokine levels in chronic bronchitic rats.METHODS:The rats were randomly divided into control group , model group and hesperidin treatment group .The rat model of chronic bronchitis was established by smoking .The pathological changes of the bronchial and lung tissues were observed by HE staining .The levels of TNF-α, IL-6 and IL-10 in the bronchoalveolar lavage fluid were analyzed by ELISA.The protein expression levels of ICAM-1 and VCAM-1 were detected by Western blot .RESULTS:Compared with model group , hesperidin treatment significantly reduced inflammatory infiltration in the bronchial and lung tissues , im-proved the integrity of the alveolar structure , and decreased the levels of TNF-αand IL-6 in the bronchoalveolar lavage flu-id, but had no effect on the IL-10 level.Moreover, the protein expression of ICAM-1 and VCAM-1 were dramatically inhib-ited after hesperidin treatment .CONCLUSION:Hesperidin decreases the levels of TNF-αand IL-6 in the bronchoalveolar lavage fluid in the rats with chronic bronchitis , which may be associated with the inhibition of ICAM-1 and VCAM-1 expres-sion.