A murine model of Th2 response induced by shrimp tropomyosin / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 233-236,241, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-606130
ABSTRACT
Objective:
To develop murine models of Th2 response induced by shrimp tropomyosin (ST).Methods:
Mice were sensitized with ST for 6 weeks. The serum antigen-special IgE (sIgE),total IgE and sIgG level,Th1/Th2 cytokines production were measured by ELISA. The basophil activation in mice was measured by flow cytometry.Results:
The intraperitoneal sensitization with ST for 6 weeks induced significant increase of serum sIgE,total IgE and sIgG (sIgG1,sIgG2a and sIgG2b) level in mice. Th2 cell response was induced and cytokines (IL-4,IL-5,IL-10 and IL-13) production increased in splenocytes stimulated by ST,while Th1 cytokine (IFN-γ) production decreased. As the markers of basophil activation,CD200R and CD41 expression also increased in response to ST.Conclusion:
The Th2 response is dominant in ST-induced anaphylaxis in mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2017
Type:
Article
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