Partial denture metal framework may harbor potentially pathogenic bacteria
The Journal of Advanced Prosthodontics
;
: 468-474, 2015.
Article
in English
| WPRIM
| ID: wpr-60626
ABSTRACT
PURPOSE:
The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time. MATERIALS ANDMETHODS:
This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity).RESULTS:
A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2.CONCLUSION:
The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacteria
/
Trees
/
DNA, Ribosomal
/
Pilot Projects
/
Polymerase Chain Reaction
/
Oral Health
/
Follow-Up Studies
/
Clone Cells
/
Actinobacteria
/
Biofilms
Type of study:
Observational study
/
Prognostic study
Limits:
Female
/
Humans
Language:
English
Journal:
The Journal of Advanced Prosthodontics
Year:
2015
Type:
Article
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