Study on the mechanism of different concentrations of celecoxib on pancreatic cancer cells resistance / 中国现代普通外科进展

ABSTRACT

Objective:Study on the inhibitory effect of celecoxib on pancreatic cancer SW1990 cells growth.Methods:The experiment was divided into two parts.(1) SW1990 cells were divided into fluorouracil group (50 μ g/mL),celecoxib group(50 μ mol/L),the combined group (celecoxib 50 μ mol/L+fluorouracil 50 μ g/mL),blank group,after 24 h culture of each group using the MTT assay SW1990 cell growth inhibition rate was detected by flow cytometry cell cycle distribution ratio;(2) were used at different concentrations (12.5,25.0,50.0 and 100.0 μ mol/L) of celecoxib,while using different concentrations above celecoxib were combined with 50 μ g/mL of fluorouracil treated SW1990 cells were treated 24 h,using real-time fluorescence polymerase chain reaction(RT-PCR) to detect differential expression Survivn RNA and detect the cyclooxygenase-2 (COX-2) enzyme-linked immunosorbent assay(ELISA),matrix metalloproteinase-14(MMP-14) expression differences.Results:Celecoxib group,fluorouracil group was significantly higher than the rate of the combination group cytostatic blank group (P＜0.05),the inhibition rate of the combination group was significantly higher than the celecoxib group and fluorouracil group (P＜0.05),celecoxib cloth inhibition rate was significantly lower than the fluorouracil group(P＜0.05);significantly higher than the proportion of cells celecoxib group,fluorouracil group,joint group of G0/G1 phase control group (P＜0.05),celecoxib group was significantly higher than the proportion of cells fluorouracil group G0/G1 phase combination group(P＜0.05),significantly higher than the proportion of cells fluorouracil group G0/G1 phase celecoxib group (P＜0.05);with the increasing concentration of celecoxib,celecoxib group,celecoxib+Survivn RNA fluorouracil group,COX-2 and MMP-14 protein levels were decreased and group differences were between statistically significant(P＜0.05),Under the condition of the same concentration of celecoxib and fluorouracil plus celecoxib group RNA survivin,COX-2 and MMP-14 protein expression levels were significantly lower than that of celecoxib group,the difference has statistical significance (P＜0.05).Conclusion:Celecoxib on the growth of pancreatic cancer SW1990 cells was significantly inhibited in a dose dependent manner,and its mechanism may be related to reduced Survivn RNA,COX-2 and MMP-14 protein expression.