Improvement of the Quality Standard for Shule Capsules / 中国药师
China Pharmacist
;
(12): 446-448,452, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-606362
ABSTRACT
Objective:
To establish the quality standard for Shule capsules.Methods:
The microscopic characteristic identifica-tion of Figwort and Radix bupleuri was performed under a microscope. The qualitative identification of fritillary, andrographispaniculata and polygonumbistorta was studied by TLC. The content of salvianolic acid B in Salvia miltiorrhiza was determined by HPLC. The chro-matographic separation was carried out by using a phenomenex synergi 4 hydro-RP 80A (250 mm × 4. 6 mm) column. The mobile phase consisted of acetonitrile-methanol-formic acid-water(1030591)with gradient elution at a flow rate of 1. 0 ml·min-1, and the injection volume was 10 μl . The detection wavelength and the column temperature was 286 nm and 20 ℃, respectively.Results:
The microscopic characteristics were obvious. The spots in TLC were clear without any interference. The linear range was 10. 001-100. 007 μg·ml-1(r=1. 0000). The average recovery was 99. 3% with RSD of 1. 8% (n=6).Conclusion:
The method is simple with high specificity and good repeatability, which can be used as the quality control method for Shule capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
/
Qualitative research
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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