Therapeutic effect and mechanism of Xifeng Dingxuan granules on focal incomplete cerebral ischemia vertigo model rats / 国际药学研究杂志

ABSTRACT

Objective To evaluate the effects of Xifeng Dingxuan granules on behavioral changes and brain energy metabo?lism on focal incomplete cerebral ischemia vertigo model rat. Methods Experimental rats were divided into normal control group, sham operation group,model group,flunarizine hydrochloride group(0.5 mg/kg),ginkgo leaf group(8 mg/kg)and Xifeng Dingxuan granule groups(0.27,0.54 and 1.08 g/kg). The drugs were all administered intragastrically. During the experimental period,step-down test was conducted to monitor the escape latency of rats from electrical stimulation,and the blood flows in right brain vestibular nucleus were measured. At the end of the experiment,the levels of the lactate(LAC),lactic acid dehydrogenase(LDH),superoxide dismutase(SOD)and malondialdehyde(MDA)in brain tissue were determined by colorimetry. Results 1. Compared with the normal control group,the escape latency of rats from electrical stimulation in model group was significantly prolonged(P<0.01). The escape latency was significantly reduced by the treatment of Xifeng Dingxuan granules(P<0.01),and the dosage of 0.54 g/kg was most effec?tive. 2. Compared with the sham operation group,the blood flow of right brain vestibular nucleus in the model group was decreased (P<0.01). The decreased blood flow could be alleviated by the treatment of Xifeng Dingxuan granules and the dosage of 0.27 g/kg was most effective(P<0.01). 3. Compared with sham operation group,the contents of LAC and LDH in the right brain tissue were signifi?cantly increased in the model group(P<0.01). Compared with the model group,the contents of LAC and LDH were decreased signifi?cantly by the treatment of Xifeng Dingxuan granules with the dosage of 0.27 g/kg and 0.54 g/kg(P<0.01). 4. Compared with the sham operation group,the content of MDA and the activity of SOD in the right brain tissue were significantly increased in the model group (P<0.01)and could be alleviated by the treatment with Xifeng Dingxuan granules at each dose(P<0.01). Conclusion The results indicated that Xifeng Dingxuan granules could suppress the decrease of blood flow in vestibular nucleus,improve the brain energy me?tabolism and reduce the oxidative stress response in focal incomplete cerebral ischemia vertigo rat model ,which implicated the possi?ble therapeutic effect of Xifeng Dingxuan granules on the vertigo.