Establishment of variables-specific reference ranges of von Willebrand factor antigen and activity / 中国输血杂志

ABSTRACT

Objective To analyze the screening results of yon Willebrand factor among patients before blood transfusion in Ruijin Hospital and discuss von Willebrand factor in ABO blood group and the relationship between age and gender,refine the classification of vWF antigen and activity by reference factors.Methods The von Willebrand factor among 247 cases of patients before blood transfusion in Ruijin Hospital with no clinical manifestations of abnormal blood clots and routine coagulation as laboratory tests for normal surgical patients.The vWFAg and vWFAct were measured by immune turbidimetric method and ABO blood group was identified by blood type serology.Furthermore,the differences between A,B,O,AB different blood groups,sex and high (≥40 years) and low age group (＜40 years) were compared by statistical methods.Results The levels of vWFAg in different blood groups were as followsA blood type98.5-142.00,B blood type97.90-160.30,O blood type82.13-125.45,A B blood type103.00-135.80.The levels of vWFAct in different blood groups were as followsA blood type76-130.14,B blood type78.06-144.3,O blood type60.89-116.11,AB blood type88.99-124.09.O blood type vWFAg and vWFAct were lower significantly (P＜0.05) than non-O blood type,the difference was.Besides,young vWFAg and vWFAct were lower significantly than in the elderly.There was no significant difference in vWFAg and vWFAct levels between male and female groups.At last,the reference range of four groups of vWF activity (antigen) was obtained.Conclusion Plasma vWF antigen and activity levels were significantly affected by ABO blood type and age,and the refined reference range established for these influencing factors was beneficial for more detailed diagnosis of VWD and predicting vWF levels associated with bleeding and thrombosis risk.