Study on Quality Standard for Herba Euphorbia Esulae / 中国药师

ABSTRACT

Objective:To establish the quality standard for Herba Euphorbia esulae. Methods:A TLC method was used to identify Herba Euphorbia esulae. An HPLC method was used to determine the content of quercetin with the following conditions an AlltimaTM C18column(150 mm ×4.6 mm,5 μm) was eluted with the mobile phase of acetonitrile-0.4％ phosphoric acid(3070), the flow rate was 1. 0 ml·min-1 , the detection wavelength was 360 nm and the column temperature was 35℃. Results:The characteristic spots of samples were the same as those of the standard samples. The linear range of quercetin was 3.194-102.208 μg·ml-1(r=0.9999) and the average recovery was 99. 0％ (RSD=1. 68％, n=6). Conclusion:The method is quick, simple and accurate, which can be used for the quality control of Herba Euphorbia esulae.