Construction and Characterization of Electrochemical Biosensor based on Endotoxin Aptameer / 中国生物工程杂志
China Biotechnology
;
(12): 46-50, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-607579
ABSTRACT
A biosensor was constructed to detect the endotoxin.The amine-terminated aptamer was immobilized on the gold electrode surface covered in advance with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA).The modification of the gold electrode was confirmed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).It was found that MPA assembly time at 6 h in the gold electrode surface to form a stable self-assembled monolayer.The biosensor has a good linear relationship with concentration of endotoxin in the range of 0.001 ~0.1 EU/ml,it is possible to be applied for quality control in the actual biological sample.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Biotechnology
Year:
2017
Type:
Article
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