3D Hydrogel Compression Model and Effect of Cyclic Compression on Osteoblast Differentiation / 中国生物工程杂志
China Biotechnology
;
(12): 8-14, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-607588
ABSTRACT
Objective:
3D hydrogel cell model was established,and cyclic compressive loading on MC3T3-E1 cell with different intensities,frequencies and durations was applied,in order to research the suitable solution about promoting the osteoblast differentiation with cyclic compression.Methods:
Cyclic compressive loading on MC3T3-E1 cell was applied with different intensity,frequency and time.After compressive loading finished,the total RNA extraction from cell-gel constructs were performed and quantified ATF4,ALP,Runx2,Osteocalcin,RANKL and RANK mRNA.Results:
RANKL and RANK mRNA expression significantly with different frequencies cyclic compressive loading (P < 0.05),and ALP mRNA (P < 0.05) and Runx2 mRNA (P < 0.01) expression significantly with different intensities and frequencies cyclic compressive loading (P < 0.05).Meanwhile,Runx2 mRNA expression with 4h significant higher than 12h (P < 0.05),and RANKL mRNA expression with 4h significant lower than 12h (P < 0.05).Conclusion:
Determine the stress intensity and frequency,1% intensity,frequency of 0.5 Hz,4 h of cyclic compression intervention could promote the growth of osteoblasts-like cells in the 3D hydrogel model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Biotechnology
Year:
2017
Type:
Article
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