Sequencing analysis of 18S ribosomal RNA of human giant intestinal fluke (Fasciolopsis rusk/) collected in Ninh Binh and Nghe An provinces

ABSTRACT

Fasciolopsis buski is common parasite in pigs. Some patients who infected by adult worms of Fasciolopsis buski in several areas, including the provinces of Ninh Binh and Nghe An were sampled. Genome of 18S ribosomal RNA with 1950 nucleotides from giant intestinal fluke (Fasciolopsis buski) was obtained using polymerase chain reaction (PCR) and sequencing approach for comparative analysis each others and with the data deposited in GenBank (L06668). The adult worms from Ninh Binh and Nghe An were morphologically identified as Fasciolopsis buski. The genetic analysis revealed that the 18S rRNA gene of the giant intestinal flukes isolated in Ninh Binh and Nghe An were similar (homogenous 100% of nucleotides) and showed nearly absolute identity with only 2 divergent nucleotides of 0.01% (2/1950 nucleotides) to the 18S rRNA of the pig Fasciolopsis buski as the data deposited in GenBank (L066608). However, 18S ribosomal RNA of the adult worms of human Fasciolopsis buski collected from the provinces of Ninh Binh and Nghe An were similar to one another