Effects of TGF-beta onthe HPC-16-induced Neoplastic Transformation of Human Cells / 대한부인종양콜포스코피학회잡지
Korean Journal of Gynecologic Oncology and Colposcopy
;
: 243-249, 1997.
Article
in Korean
| WPRIM
| ID: wpr-60859
ABSTRACT
Human epithelial cell line immortalized with Ad12-SV40 hybrid virus was transfected with plasmid containing HPV-16 gene. Among these clones, clone-3 and clone-6 showed neoplastic transformation properties of contact inhibition, anchorage independence and cellular adhesion after 7 subcultures. The results suggest that SV40 gene in the immortalized human cell system be in concert with HPV-16 in the process of neoplastic transformation of human cells. While TGF-Beta1(5ng/ml) inhibited growth of contml cells and clone-1 cells which did not show transformation, there was no significant change on the growth of clone-3 cells with transformation properties. When transcriptional level of fibronectin on control cells and clone-3 cells were analyzed with northern blot technique, transcription of fibronectin an clone-3 cells were higher, as compared with control cells. RNA hybridization techniques were performed to compare trasnscriptional levels of TGF-Beta1 between control cells and clone-3 cells. RNA level on clone-3 cells with transformation properties was higher than on control cells. These studies indicate that TGF-Beta1 is associated with increases of fibronectin, which may lend to changes of TGF-Beta receptor and loss of its inhibitory action on the transformed cells. Thus, it seems that loss of inhibitory action of TGF-Beta which is mediated by changes of fibronectin may account for a possible mechanism of action in the HPV-16 induced transformation of human cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
RNA
/
Blotting, Northern
/
Transforming Growth Factor beta
/
Fibronectins
/
Clone Cells
/
Receptors, Transforming Growth Factor beta
/
Contact Inhibition
/
Epithelial Cells
/
Human papillomavirus 16
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Gynecologic Oncology and Colposcopy
Year:
1997
Type:
Article
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