Protective effects of Xuebijing on endothelium in sepsis-induced acute kidney injury / 解放军医学杂志

ABSTRACT

Objective To explore the protective effect of Xuebijing on the endothelium and extracellular matrix in sepsisinduced acute kidney injury (AKI) rats for providing a new clinical treatment strategy.Methods The method of cecal ligation and puncture (CLP) was used to duplicate severe sepsis model.Thirty healthy male SD rats were randomly divided into 3 groupsSham group (n=10),NS group (normal saline 4ml/kg,n=10),Xuebijing group (Xuebijing 4ml/kg,n=10).6h after CLP,the rats were sacrificed and their kidneys were resected and histopathological characteristic was observed by light microscopic and transmission electron microscopic techniques.The expressions ofET-1 mRNA,iNOS mRNA,MMP-9 mRNA,TIMP-1 mRNA in the renal tissues were measured semiquantitatively by reverse transcription-polymerase chain reaction (RT-PCR).Results The histopathological changes in renal tissue were observed by light microscope.The changes of renal glomerulus and renal tubuli in Xuebijing group were better than NS group.The ultrastructural changes in renal tissue were observed under electron microscope.Compared with NS group,ultrastructural changes of renal glomerulus and proximal convoluted tubule were smaller in Xuebijing group.The expressions of ET-1mRNA (0.631 ± 0.169vs 0.770 ± 0.154,P＜0.05),iNOS mRNA (0.507 ± 0.071 vs 0.587 ± 0.073,P＜0.05),MMP-9mRNA (0.641 ± 0.082 vs 0.742 ± 0.116,P＜0.05) and TIMP-1 mRNA (0.434 ± 0.052 vs 0.520 ± 0.049,P＜0.01) were significantly lower in XBJ group renal tissues than in NS group.The expressions of ET-1 mRNA(0.770 ± 0.154 vs 0.394 ± 0.105,P＜0.01),iNOS mRNA (0.587 ± 0.073 vs 0.326 ± 0.085,P＜0.01),MMP-9 mRNA (0.742 ± 0.116 vs 0.356 ± 0.055,P＜0.01) and TIMP-1 mRNA (0.520 ± 0.049 vs 0.351 ± 0.041,P＜0.05) in renal tissues were more significantly increased in NS group compared with sham group.Conclusions Xuebijing could reduce the levels of ET-1,iNOS,MMP-9 and TIMP-1 mRNA,protect the stability of endothelium and extracellular matrix,and reduce tissue injury in septic rats.