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Simultaneous determination of five nucleosides in Houtoujun Tablets by RP-HPLC / 中成药
Article in Zh | WPRIM | ID: wpr-609639
Responsible library: WPRO
ABSTRACT
AIM To establish an RP-HPLC method for the simultaneous content determination of five nucleosides in Houtoujun Tablets (mycelia of Hericium erinaceum).METHODS The analysis of aqueous extract of this drug was performed on a 26 ℃ thermostatic Zorbax SB-Aq C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1% phosphoric acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.RESULTS Cytidine,adenine,uridine,adenosine and guanosine showed good linear relationships within the ranges of 0.007-0.169,0.005-0.115,0.032-0.797,0.032-0.792 and 0.004-0.103 μg,whose average recoveries (n =6) were 99.4% (RSD =2.0%),98.3% (RSD =1.8%),98.0% (RSD =2.8%),101.4% (RSD =1.9%) and 101.7% (RSD =1.2%),respectively.CONCLUSION The contents of five nucleosides in Houtoujun Tablets from different manufacturers exhibit obvious differences,to which we should pay attention.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article