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The influence of thyroid hormones on the expression of NGF and BDNF after cerebral ischemia-reperfusion injury in rats / 重庆医学
Chongqing Medicine ; (36): 2030-2033, 2017.
Article in Zh | WPRIM | ID: wpr-610091
Responsible library: WPRO
ABSTRACT
Objective To investigate the neuroprotective effect of thyroid hormones T3 on cerebral ischemia-reperfusion injury in rats and its mechanism.Methods SD rats were divided into four groups:sham+saline group,sham+T3 group,MCAO+saline group,MCAO+T3 group.The cerebral ischemia-reperfusion injury rat models were established by right middle cerebral artery occlusion.Thyroid hormones(10 μg/100 g)or normal saline were given respectively by intraperitoneal injection twice at 1 h after the onset of ischemia and 6 h after reperfusion.Neurobehavioral score was evaluated at 24 h after reperfusion;TTC staining was used to label infarction area;RT-PCR was used to detect the mRNA level of nerve growth factor(NGF)and brain derived neurotrophic factor(BDNF)in brain tissue;Western blot was employed to determine alterations in protein levels of NGF and BDNF.Results Compared with MCAO+saline group,the neurological deficit and the volume of cerebral infarction of MCAO+T3 group was decreased,and the mRNA and protein expression of NGF and BDNF of MCAO+T3 group were increased(P<0.05).Conclusion Thyroid Hormones could promote the nerve repair,stimulate the nerve regeneration and improve the nervous behavioral function by up-regulating the expression of NGF and BDNF.
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Full text: 1 Index: WPRIM Type of study: Prognostic_studies Language: Zh Journal: Chongqing Medicine Year: 2017 Type: Article
Full text: 1 Index: WPRIM Type of study: Prognostic_studies Language: Zh Journal: Chongqing Medicine Year: 2017 Type: Article