Determination of Amidozon and Amantadine Hydrochloride in Compound Amantadine Hydrochloride Tablets by HPLC-ELSD / 中国药师
China Pharmacist
;
(12): 931-932, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-610154
ABSTRACT
Objective:
To establish a method for the determination of amidozon and amantadine hydrochloride in compound amantadine hydrochloride tablets by HPLC-ELSD.Methods:
The analytical column was an Agilent Eclipse Plus C18 column (250 mm×4.6 mm, 5 μm).The mobile phase consisted of 0.1% trifluoroacetic acid solution-acetonitrile (85∶15) and the flow rate was 0.7 ml · min-1.The column temperature was room temperature.Both evaporation temperature and atomization temperature were 40 ℃.The flow rate of carrier gas (nitrogen) was 1.8 L · min-1 and the injection volume was 10 μl.Results:
The peak area and logarithm of injection volume of ampicrine showed a good linear relationship within the range of 606.72-3 033.60 μg · ml-1 (r=0.999 7), and that of amantadine hydrochloride showed a good linear relationship within the range of 400.96-2 004.80 μg·ml-1 (r=0.999 4).The average recovery was 99.5% and 99.3%, and RSDs was 0.35% and 0.40%(n=9), respectively.Conclusion:
The method is simple, rapid, accurate and reproducible, and suitable for the determination of aminopyrine and amantadine hydrochloride in compound amantadine aminopyrine tablets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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