Effect of total flavonoid-containing serum of Pollen Typhae on autophagy of macrophages and its effect on Akt/mTOR signaling pathway / 实用医学杂志

ABSTRACT

Objective To study the effect of total flavonoids of Pollen Typhae on the autophagy of macro-phages and its effect on Akt/mTOR signaling pathway. Methods To observe the effect of serum containing drugs on the proliferation of SD rats. The serum containing 20%of the drug-containing serum and the macrophages were selected by the method of MMT. Interleukin(IL-10),interferon-γ(IFN-γ)and autophagy-related genes Akt and mTOR mRNA and protein were detected. Results There was no significant difference between the OD value of TYTF serum in the concentration range of 10% ~ 40% and so as the OD value of normal control(P > 0.05). The expression of autophagosomes in each dose group of TYTF was significantly increased under TEM. The autophagic expression was not typical in the normal control group. The expression of Beclin1 protein in TYTF group was signifi-cantly higher than that in normal control group(P 0.05). The expression of Beclin1 mRNA in TYTF group was significantly higher than that in normal control group (P < 0.05),while the expression of Akt and mTOR mRNA was significantly lower than that of normal control group (P < 0.05). The expression of IL-10 in TYTF group was significantly higher than that in normal control group (P < 0.05). The expression of IFN-γ was significantly lower than that of normal control group(P<0.05). Conclusions The total flavonoid serum has anti-atherosclerotic effect. The mechanism may be related to inhibition of the activation of Akt/mTOR signaling path-way,including macrophage autophagy activity induction ,plaque macrophage infiltration reduction and inflamma-tory response factors secretion inhibition ,inflammatory response activities inhibition. Thereby it can promote atherosclerosis plaque stability of the purpose of vulnerability.