The expression of microRNA-21,microRNA-135b and microRNA-141 in colon cancer tissue and their correlation / 国际检验医学杂志

ABSTRACT

Objective To investigate the expression level of microRNA-21,microRNA-135b and microRNA-141 in colon cancer tissue.Methods Totally 40 cases of colon cancer specimen from surgical resection in our hospital from May 2013 to April 2014 were selected,40 healthy colon tissues from people who entered our hospital at the same period for physical examination were selected.The expression level of microRNA-21,microRNA-135b and microRNA-141 were determined using real time PCR.Chosed sex,age,lymph node metastasis,TNM staging,differentiation degree as related factors by literatures published in home and abroad,assessed the correlation between the level of microRNA-141,microRNA-135b,microRNA-21 and its expression in colorectal cancer.Results The expression level of microRNA-21,microRNA-135b and microRNA-141 in colon cancer tissues were remarkably higher than those in healthy tissues,the difference was statistically significant(P<0.05).The expression levels of microRNA-141,microRNA-135b and microRNA-21 in colon cancer tissues were closely related to the occurrence of lymph node metastasis,progression and stage of colon cancer,and the difference was statistically significant(P<0.05).Conclusion The expression of microRNA-21,microRNA-135b and microRNA-141 are upregulated in colon cancer tissue,which is highly correlated with the lymphatic metastasis and progressiveness of colon cancer.