The expression and significance of HMGB1,TLR4 and STAT3 in mousemodel of anti glomerular basement membrane glomerulonephritis / 国际检验医学杂志

ABSTRACT

Objective To study the expression and significance of HMGB1,TLR4 and STAT3 in the mouse model of anti glomerular basement membrane glomerulonephritis.Methods Totally 30 mice were randomly divided into normal group(control group),anti GBM glomerulonephritis model group(experimental group),PAS staining was used to observe the changes of glomerular basement membrane,HMGB1,p-STAT3 mRNA in renal tissue were detected by RT-PCR,HMGB1,TLR4,STAT3,p-STAT3 in renal tissue was detected by Western blot method.Results Compared with the control group,the experimental group of mice glomerular basement membrane thickening;the relative expression of HMGB1,TLR4,STAT3,p-STAT3 and HMGB1 mRNA,p-STAT3 mRNA in the kidney tissue of experimental group were significantly higher than the control group,the difference was statistically significant(P<0.05).The expression level of HMGB1 and TLR4,TLR4 and p-STAT3,HMGB1 and p-STAT3,HMGB1 mRNA and p-STAT3 mRNA in renal tissues of mice in experimental group,there were a positive correlation(r=0.401,P=0.005;r=0.399,P=0.005;r=0.412,P=0.004;r=0.398,P=0.005).Conclusion The inflammatory effect of HMGB1 in mice with anti glomerular basement membrane glomerulonephritis can activate STAT3 by binding to its receptor TLR4,so as to achieve anti glomerular basement membrane glomerulonephritis.