Study on Effect of Ropivacaine on Migration of Breast Cancer Cells / 现代检验医学杂志

ABSTRACT

Objective To observe the different behavior of migration in breast cancer cells,when exposure to ropivacaine of different concentrations.Methods The breast cancer cells MDA-MB-231 were inoculated into culture medium.They were divided into four groupslow-dose group (150 μg/ml,A group),medium-dose group (250 μg/ml,B group),high-dose group (450 μg/ml,C group) and control group (D group),and detected the cell proliferation,abilities of cell scratch and chemotaxis.Results MDA-MB-231 cell viability of B and C group was significantly lower than that of D group (t=8.845～11.654,P＜0.05).Migration and chemotaxis abilities of B group and C group were decreased than those of D group (t=6.054～ 12.412,t=8.654～10.214,P＜0.05),and there was no difference between A group and D group (t=1.025～1.124,P＞ 0.05).Conclusion Ropivacaine could restrain the viability and migration abilities of breast cancer cells depending on its different concentrations.