Clinical Significance of Serum Kynurenine and Uric Acid Levels Detection in Chronic Myeloid Leukemia Patients / 现代检验医学杂志

ABSTRACT

Objective To investigate the clinical significance of kynurenine (KYN) and uric acid (UA) levels in chronic myeloid leukemia patients (CML).Methods 30 CML patients (CML group) and 25 healthy controls (Control group) were enrolled in the hospital from Mar 2014 to Dec 2015.Serum KYN and tryptophan (TRP) of all subjects were determined by high performance liquid chromatography,and KYN/TRP ratio (KTI) were calculated.Leukocytes were counted by automat ic blood cell analyzer.Serum UA were detected by enzyme method.Correlation between UA and KTI,leukocytes were analyzed by Pearson correlation.Results The serum KTI of the CML group (49.76±19.06) before treatment was significantly higher than that of the control group (27.87-±-5.06,t=4.470,P=0.001).KTI cutoff value was 40 by ROC analysis.There had correlation between KTI and leukocytes (r=0.743,P=0.001) and no correlation between KTI and ages (r=0.205,P =0.276).KTI (64.7±17.8) and the leukocytes [(96.7±64.1) × 109/L] of 10 cases of KTI≥40 of CML patients before treatment was significantly higher than that of KTI (27.0± 10.9,t=7.102,P =0.001) and the leukocytes[(10.1 ± 5.2) × 109/L,t=4.285,P=0.002] after treatment.The serum UA in KTI＜40 CML patients (n=15,354.98±103.9 μmol/L) was significantly lower than that of KTI≥40 CML group CML (n=15,484.98±132.1 μmol/L,t=1.432,P=0.045).The UA in CML group was associated with KTI (r=0.573,P=0.001) and leukocytes (r=0.537,P=0.002).Conclusion The increase of KTI in CML patients suggested that an increased activity of indoleamine-2,3-dioxygenase (IDO).IDO activity may associated with CML morbidity and therapy effect.The detection of KTI may contribute to the diagnosis of CML and assessing therapy effect of CML.