Apoptosis of human hepatocellular carcinomaHepG-2 cells induced by total flavonoids of Verbena officinalis L through targeting topoisomerase Ⅱ / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 1147-1152, 2017.
Article
in Zh
| WPRIM
| ID: wpr-613658
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WPRO
ABSTRACT
Aim To explore whether total flavonoids of Verbena officinalis L(TFV)can induce apoptosis of HepG-2 cells through targeting topoisomerase Ⅱ.Methods HepG-2 cells were cultured with TFV at 200,100,50 mg·L-1.Cell apoptosis was evaluated by TUNEL-DAPI double staining.TopⅡ activity was detected by supercoiled pBR322 DNA relaxation assay.The levels of the mRNA of TOP Ⅱα,TOP Ⅱβ were analyzed by real time PCR.Expression of Bcl-2,Bax,TOP Ⅱα,TOP Ⅱβ and caspase-3 was analyzed by Western blot.
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WPRIM
Language:
Zh
Journal:
Chinese Pharmacological Bulletin
Year:
2017
Type:
Article