Presence of intrinsic myocardial systolic and diastolic dysfunctions at early stage of sepsis in mild CLP rats / 中国病理生理杂志

ABSTRACT

AIM:To observe the time pattern of intrinsic myocardial dysfunction and other organ dysfunction in a rat model of mild cecal ligation and puncture (CLP).METHODS:Male SD rats were randomly divided into sham group and CLP group.At 6 h, 9 h and 12 h after sham operation or CLP, intrinsic myocardial systolic and diastolic functions were determined by Langendorff system.The expression of cardiac tumor necrosis factor (TNF)-α, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was measured.In addition, serum activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as well as blood urea nitrogen (BUN) were analyzed.For evaluating pulmonary edema, the lung wet-dry weight (W/D) ratio was calculated.RESULTS:In mild CLP rats, the mortality rate was 26.7% on day 10 after CLP.At 9 h and 12 h after CLP, the maximum rate of positive and negative changes in left ventricular pressure (±dp/dt) were decreased in CLP group compared with sham group.At 6 h after CLP, cardiac mRNA expression of TNF-α, ICAM-1 and VCAM-1 was increased in septic rats compared with the controls.At 9 h after CLP, cardiac protein levels of TNF-α and VCAM-1 were higher in CLP group than that in sham group (P<0.05).The serum AST activity at 9 h and ALT activity at 12 h after CLP were increased in CLP group compared with sham group.However, no difference in BUN and lung W/D ratio at 6 h, 9 h and 12 h between CLP group and sham group was observed.CONCLUSION:Our findings highlight the presence of intrinsic myocardial dysfunction at 9 h after CLP in a rat model of mild CLP.Intrinsic myocardial dysfunction occurs earlier than the liver and kidney dysfunctions.