Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients
Journal of the Korean Surgical Society
;
: 111-115, 2010.
Article
in Korean
| WPRIM
| ID: wpr-61414
ABSTRACT
PURPOSE:
Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray.METHODS:
DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (+) patients (MF=152, mean age 35 years, 22~59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip.RESULTS:
Fourteen patients (9 HIV (+), 5 HIV (-)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (-) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV.CONCLUSION:
Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (-) patients. HPV types on HIV (+) male anal condyloma are similar to those detected in the Korean female uterine cervix.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Cervix Uteri
/
Chimera
/
Polymerase Chain Reaction
/
HIV
/
Oligonucleotide Array Sequence Analysis
/
Coinfection
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Language:
Korean
Journal:
Journal of the Korean Surgical Society
Year:
2010
Type:
Article
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