Liposome-encapsulated brain-derived neurotrophic factor targets blood-brain barrier / 中国免疫学杂志

ABSTRACT

Objective:To observe the characteristics of the tissue distribution of the blood brain barrier through the modification of the liposome encapsulated brain derived neurotrophic factor.Methods: A total of 32 SD rats were selected from Xi′an Medicine College of Foreign Affairs as the research object,and randomly divided into two groups (n=16) and control group (n=16).To establish a rat model of AD rats in the control group received no treatment,liposome group by rat tail vein injection of 10 μg/kg Tf-pGFAP-BDNF-PEG,analysis of liposome entrapped BDNF targeting liposomes through blood brain barrier tissue distribution characteristics.Results: 40-50 nerve growth factor (1 ml) was collected by using 24mCi99mTC and 20 L 0.5 ml was collected on the top of the column.The concentration of 8-17 tube in the peak was recovered,and the results showed that the labeling rate of neurotrophic factor was 98.9％.Liposome group for tenth days,14 days of BDNF immunoreactive cells,significantly higher than the control group (P<0.05);neurotrophic factor in brain tissue of rats in body lipid gene expression at day fourteenth,significantly higher than the control group (P<0.05);expression of neurotrophic factors in liposome group rat,and neurotrophic factor expression level was significantly higher than the control group (P<0.05).The cerebrospinal fluid was clear,colorless and transparent,and no red blood cells were detected under microscope.Liposome group in cerebrospinal fluid of rats with fecal occult blood detection results of gold colloid showed negative,while the control group was positive.Conclusion: The combination of transferrin and polyethylene glycol modified liposomes combined with brain specific promoter can improve the targeting of liposomes.